X-Ray Visualization of Intraductal Ethanol-Based Ablative Treatment for Prevention of Breast Cancer in Rat Models

There are still a limited number of primary interventions for prevention breast cancer. For women at high risk developing cancer, the most effective intervention is prophylactic mastectomy. This drastic surgical procedure in which mammary epithelial cells that can give rise to cancer completely removed along with surrounding tissue. The goal this protocol demonstrate feasibility minimally invasive intraductal could become new prevention. local would preferentially ablate before they malignant. Intraductal methods deliver solutions directly these rodent models have been developed Michigan State University and elsewhere. rat gland consists single ductal tree has simpler more linear architecture compared human breast. However, chemically induced offer valuable tools proof-of-concept studies preventive scalability from mouse humans. Here, delivery an ethanol-based ablative solution containing tantalum oxide nanoparticles as X-ray contrast agent ethyl cellulose gelling into described. Delivery aqueous reagents (e.g., cytotoxic compounds, siRNAs, AdCre) by injection described previously models. description emphasizes methodological changes steps pertain uniquely delivering solution, formulation consideration minimize systemic side effects imaging vivo assessment filling. Fluoroscopy micro-CT techniques enable determine success extent filling thanks compatibility tantalum-containing agent.